All adenoviruses (Ads) sequenced so far encode a single endopeptidase of the cysteine class, named adenain. The Ad2 adenain is a 204-residue, nearly inactive monomer, which is activated during virus maturation by an 11-residue cleavage fragment of capsid protein pVI. This chapter describes the synthesis, purification, activation, and assay of recombinant human Ad type adenain.
CITATION STYLE
Weber, J. M. (2007). Synthesis and assay of recombinant adenovirus protease. Methods in Molecular Medicine, 131, 251–255. https://doi.org/10.1007/978-1-59745-277-9_18
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