Squamous cell carcinoma is the most common cancer in the oral cavity. DNA tests showed that almost 90% of cases revealed wild p53 gene mutations. Wild p53 gene mutations cause p53 inactivation so the cell cycle does not stop in G1 phase but continues to S phase and G2 and M, it makes the mutated DNA remains multiplied and apoptosis does not occur. One candidate of the cancer treatment alternatives is pomegranate extract (Punica granatum L – PGL). Purpose: The purpose of study was to examine the effect of PGL on wild p53 expression in oral cavity malignant cell of swiss webster strain mice. Methods: Thirty- two swiss webster strain mice (Balb/c) 5 months old were randomly divided into four groups. Two control groups (K0: no benzopirene exposed and untreated; K1: benzopirene exposed and untreated); and 2 treatment groups (P1: benzopirene exposed and given EA; P2: benzopirene exposed and given PGL extract). The expression of wild p53 was determined by immunohistochemical techniques. results: The results showed that administration of PGL could increase the expression of wild p53 in malignant epithelial cells in the oral mucosa of mice, and the expression was higher than EA. Conclusion: This study suggested that the PGL extract could express wild p53 in the oral cavity malignant cells of swiss Webster strains mice.
Hernawati, S., Rantam, F. A., Sudiana, I. K., & Rahayu, R. P. (2013). Efek ekstrak buah delima (Punica Granatum L) terhadap ekspresi wild p53 pada sel ganas rongga mulut mencit strain swiss webster. Dental Journal (Majalah Kedokteran Gigi), 46(3), 148. https://doi.org/10.20473/j.djmkg.v46.i3.p148-151