Thiosulfate induced cells of Paracoccus denitrificans GB17 oxidize thiosulfate and sulfide to sulfate. A mutant carrying a Tn5-mob insertion in the soxB gene is unable to oxidize thiosulfate or sulfide suggesting a linkage of both activities. To test this assumption we have separated the components of the thiosulfate-oxidizing enzyme system of the wild-type by ion exchange chromatography. The SoxB protein coeluted with a highly active sulfide dehydrogenase. Analysis by polyacrylamide gel electrophoresis revealed one major protein of Mr 32k. Thus, the SoxB protein appeared to be identical with sulfide dehydrogenase. © 1994.
Schneider, A., & Friedrich, C. (1994). Sulfide dehydrogenase is identical with the SoxB protein of the thiosulfate-oxidizing enzyme system of Paracoccus denitrificans GB17. FEBS Letters, 350(1), 61–65. https://doi.org/10.1016/0014-5793(94)00732-2