Detection of fluorescence dye-labeled proteins in 2-D gels using an Arthur™ 1442 multiwavelength fluoroimager

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Abstract

Labeling of proteins with SYPRO® Orange, SYPRO Red, and SYPRO Ruby after 2-D polyacrylamide gel electrophoresis (PAGE) using plastic-backed immobilized pH gradient (IPG) strips and precast SDS polyacrylamide gels was tested. Protein spots were detected using an Arthur™ 1442 Multiwavelength Fluoroimager. The labeling methods described allow detection of proteins both after isoelectric focusing (IEF) and PAGE with a sensitivity higher than or comparable to standard silver staining methods. In addition to the post-labeling methods mentioned above, pre-labeling with the cysteine-specific fluorophore monobromobimane before 2-D PAGE is a sensitive, fast, and cost-effective alternative to existing staining protocols.

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Herick, K., Jackson, P., Wersch, G., & Burkovski, A. (2001). Detection of fluorescence dye-labeled proteins in 2-D gels using an ArthurTM 1442 multiwavelength fluoroimager. BioTechniques, 31(1), 146–149. https://doi.org/10.2144/01311pf02

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