Applications of immobilized metal affinity electrophoresis

Abstract

Immobilized metal affinity electrophoresis (IMAEP) is a technique in which metal ions are integrated in a localized polyacrylamide gel section and which possess an insignificant electrophoretic migration. IMAEP has been implemented into both native and sodium dodecyl sulfate (SDS) one-dimensional (1-D) and two-dimensional (2-D) polyacrylamide gel electrophoresis (PAGE) systems. The gel piece containing metal ions is overlaid on top of an individual well in 1-D IMAEP, and gel strip is overlaid on top of the two-second dimensional polyacrylamide gel in 2-D IMAEP, respectively. This method is used to capture/enrich metal ion binding proteins. Due to the preferential binding between iron (iii), manganese (ii), or aluminum (iii) ions and the phosphate group, these metal ions are used to capture/enrich phosphoproteins from a mixture of proteins. Data shows that SDS not only does not interfere with the extraction of phosphoproteins but also help unmasking phosphate groups by unfolding the phosphoproteins to facilitate metal ion-phosphate binding while supplying the protein with negative charges. In addition, IMAEP together with avidin functional affinity electrophoresis (AFAEP) and antibody affinity electrophoresis (AAEP) have been used to demonstrate the separation of proteins by their functionalities. AFAEP is used here to capture biotinylated proteins using avidin and AAEP is used here to capture protein G using IgG.