We have cloned and sequenced a cDNA encoding GTP-binding protein from a fish cell, CHSE-214. The clone was 1493 bp long and contained an open reading frame encoding 364 amino acids. It has the five sequence motifs G1-G5 that are conserved in all GTP-binding proteins. Its amino acid sequences are strikingly different from those of the well-characterized G-proteins. However, sequences closely related to this protein are found in various kinds of species including human, Arabidopsis, Drosophila and archaebacteria, suggesting a novel subfamily within the superfamily of the GTP-binding proteins. Northern analysis indicates that this gene is constitutively expressed at a low level in normal cells but is induced by fish rhabdovirus infection at about 24 h post infection and disappears thereafter. Based on these observations, we propose that this protein represents an evolutionarily conserved novel subfamily of GTP-binding proteins which may play an important role in fish rhabdovirus infection.
CITATION STYLE
Lee, E. H., Kim, H. J., Park, J. J., Choi, J. Y., Cho, W. J., Cha, S. J., … Park, J. W. (1998). Molecular cloning of a novel GTP-binding protein induced in fish cells by rhabdovirus infection. FEBS Letters, 429(3), 407–411. https://doi.org/10.1016/S0014-5793(98)00641-3
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