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Journal Article

Application of fluorescence polarization in HTS assays.

Methods in molecular biology (Clifton, N.J.) (2009) 565 127-143
DOI: 10.1007/978-1-60327-258-2_6
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Abstract

Steady-state measurements of fluorescence polarization have been widely adopted in the field of high-throughput screening for the study of biomolecular interactions. This chapter reviews the basic theory of fluorescence polarization, the underlying principle for using fluorescence polarization to study interactions between small-molecule fluorophores and macromolecular targets, and representative applications of fluorescence polarization in high-throughput screening.

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Huang, X., & Aulabaugh, A. (2009). Application of fluorescence polarization in HTS assays. Methods in Molecular Biology (Clifton, N.J.), 565, 127–143. https://doi.org/10.1007/978-1-60327-258-2_6

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