Pan-specific anti-acetylated lysine antibody is a valuable tool to detect, validate, and quantify protein lysine acetylation. Compared to site-specific acetyllysine antibodies that are not readily available in most cases, polyclonal pan-specific acetyllysine antibodies can be generated with synthetic antigen of a carrier protein that has its lysine residues chemically acetylated. Here, we describe protocols of synthesizing acetylated ovalbumin (OVA), immunizing rabbits, and characterizing pan-specific polyclonal anti-acetylated lysine antibodies. © Springer Science+Business Media, LLC 2013.
CITATION STYLE
Xu, W., & Zhao, S. (2013). Generation and characterization of pan-specific anti-acetyllysine antibody. Methods in Molecular Biology, 981, 137–150. https://doi.org/10.1007/978-1-62703-305-3_11
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