Protein profiling of coloring rice (Oryza sativa L.) using SDS-PAGE and experion TM 260 analysis

Abstract

Black rice is one of coloring rice and contains high nutrients, minerals, and bioactive compounds. This study provides to investigate the protein profile of rice by using SDS PAGE and Experion TM 260. Samples were collected from East Java, Central Java and West Java, there were white rice (WREJ), red rice (RREJ), black rice from East Java (BREJ), Central Java (BRCJ) and West Java (BRWJ). Proteins were extracted from rice seeds and run with SDS-PAGE and ExperionTM260. The molecular weight of the protein was determined by the Rf value. Fifteen protein bands were detected on each sample whereas based on ExperionTM260. The range of proteins detected on the SDS page was 8-137kDa, whereas ExperionTM260 detected protein bands from 1.2kDa to 260kDa. There was one protein in WREJ (127kDa), two proteins in RREJ (132kDa & 18kDa) and two proteins in BRWJ (25kDa &21kDa). Whereas the other proteins were not the same in each sample in both analyzes. Several proteins with MW more than 137kDa and less than 8kDa were identified using ExperionTM260, not on SDS-PAGE with CBB stain analyses. Our study suggested that several proteins bands were detected only on specific rice and it could be as biochemical markers for further research.