Whole genome amplification is required to ensure the availability of sufficient material for copy number variation analysis of a genome deriving from an individual cell. Here, we describe the protocols we use for copy number variation analysis of non-fixed single cells by array-based approaches following single-cell isolation and whole genome amplification. We are focusing on two alternative protocols, an isothermal and a PCR-based whole genome amplification method, followed by either comparative genome hybridization (aCGH) or SNP array analysis, respectively.
CITATION STYLE
Dimitriadou, E., Esteki, M. Z., & Vermeesch, J. R. (2015). Copy number variation analysis by array analysis of single cells following whole genome amplification. In Whole Genome Amplification: Methods and Protocols (pp. 197–219). Springer New York. https://doi.org/10.1007/978-1-4939-2990-0_14
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