Tannerella forsythia, a gram-negative anaerobic bacterium, is one of the most important pathogens in periodontal disease. However, it has been difficult to construct a gene-deletion mutant in this organism, which may serve as a useful tool in microbiological research. We reported a highly efficient method to construct a gene-deletion mutant of T. forsythia in 2007, and it was accomplished by preparing competent cells from a colony grown on an agar medium instead of a broth culture. Here, we describe the same method with some improvements.
CITATION STYLE
Nagano, K., & Hasegawa, Y. (2021). Construction of a Gene-Deletion Mutant in Tannerella forsythia. In Methods in Molecular Biology (Vol. 2210, pp. 25–31). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0939-2_3
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