In this paper, we present the results of a ligand- and structure-based virtual screen targeting LRRK2, a kinase that has been implicated in Parkinson's disease. For the ligand-based virtual screen, the structures of 12 competitor compounds were used as queries for a variety of 2D and 3D searches. The structure-based virtual screen relied on homology models of LRRK2, as no X-ray structure is currently available in the public domain. From the virtual screening, 662 compounds were purchased, of which 35 showed IC50 values below 10 μM in wild-type and/or mutant LRRK2 (a hit rate of 5.3%). Of these 35 hits, four were deemed to have potential for medicinal chemistry follow-up.
Gancia, E., De Groot, M., Burton, B., & Clark, D. E. (2017). Discovery of LRRK2 inhibitors by using an ensemble of virtual screening methods. Bioorganic and Medicinal Chemistry Letters, 27(11), 2520–2527. https://doi.org/10.1016/j.bmcl.2017.03.098