Validation of a method for quantification of Lead, Chromium, Magnesium, Zinc &copper in human blood and serum using Atomic Absorption Spectrometry

Abstract

The quality of analytical data coming from the analysis of biological samples like blood, serum and human hair is of fundamental importance for judging the limit of contaminants. Since this analysis is exposed to errors due to sampling, sample preparation and instrument, it is important to validate the analytical methods used for such measurements. A method for quantification of lead, chromium, copper, magnesium and zinc in blood and in serum using electrothermal and flame atomic absorption spectrometer was established. Full validation was done to ensure the suitability of the developed method for the purpose of analysis. The main parameters evaluated in the validation study were the limit of detection (LOD), limit of quantification (LOQ), linearity range, accuracy, precision and expanded uncertainty of the measurement results of in case of each element. The results obtained revealed that the analytical method is valid for the analysis of biological samples.