Isolation and identification of some bacteria from imported meat (beef burger) by using vitek2 technique

Abstract

Iraqi's local markets contains a significant variety of imported meats from different sources, beef burger is one of them, the current study aimed to knowledge occurrence and prevalence of bacteria in meat of beef hamburger by using vitek 2 technique in Al-Diwaniyah city. Forty Samples of beef burger were chosen randomly from local markets, all the isolates submitted to culture on many of media agar like MacConkey, blood base agar, SS agar, manitol salt agar, Eosin-methylene blue agar, Chromagar Orientation, , and salmonella Chromagar, then the isolates tested by vitek 2 technique to confirm final diagnosis, the study was shown there are many of bacteria reside in beef hamburger at different percentage, E.coli (55%), Klebsiella pneumonia (38%), Klebsiella oxytoca (2%), Staphylococcus aureus (44%), Proteus mirabilis (13%), Salmonella typhimurium (18%), Enterococcus feacalis (8%) and serratia marcescens (3%). It could consideration meat of beef burger is an eligible nutrient to the multiplication of many of bacteria types that may be very dangerous if it transmitted to human.