A method for direct detection of Listeria monocytogenes in 25 ml of raw milk is presented. The detection limit can be situated between 10 and 5 CFU. The detection method is based on chemical extraction of the milk components and PCR amplification with two nested pairs of primers specific for Listeria monocytogenes.
CITATION STYLE
Herman, L. M. F., De Block, J. H. G. E., & Moermans, R. J. B. (1995). Direct detection of Listeria monocytogenes in 25 milliliters of raw milk by a two-step PCR with nested primers. Applied and Environmental Microbiology. American Society for Microbiology. https://doi.org/10.1128/aem.61.2.817-819.1995
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