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Direct detection of Listeria monocytogenes in 25 milliliters of raw milk by a two-step PCR with nested primers

Applied and Environmental Microbiology
DOI: 10.1128/aem.61.2.817-819.1995
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Abstract

A method for direct detection of Listeria monocytogenes in 25 ml of raw milk is presented. The detection limit can be situated between 10 and 5 CFU. The detection method is based on chemical extraction of the milk components and PCR amplification with two nested pairs of primers specific for Listeria monocytogenes.

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Herman, L. M. F., De Block, J. H. G. E., & Moermans, R. J. B. (1995). Direct detection of Listeria monocytogenes in 25 milliliters of raw milk by a two-step PCR with nested primers. Applied and Environmental Microbiology. American Society for Microbiology. https://doi.org/10.1128/aem.61.2.817-819.1995

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