Application of the yeast one-hybrid technique to plant functional genomics studies

Abstract

The yeast one-hybrid technique could help analyze DNA–protein interactions through the expression of reporter genes. It has become a frequently used molecular method in recent years. This mini-review briefly introduces the mechanism, the technical procedure, the major advantages and disadvantages as well as the application of the yeast one-hybrid technique to plant functional genomics studies. Prospects for future development are put forward.