Rocket Immunoelectrophoresis Technique or Electroimmunodiffusion

Abstract

The rocket immunoelectrophoresis technique or electroimmunodiffusion (EID) (1) is a simple, fast, and reproducible technique for quantitation of a single protein, and is also applicable in a protein mixture. Several unknown samples can be analyzed on a single plate. Known reference solutions have to be included in each plate. To obtain an accurate quantitation, the proteins in the reference solutions and in the unknown samples have to be physicochemically and immunologically identical. The samples are applied in wells punched out of an agarose gel containing the corresponding monospecific antiserum. One-dimensional electrophoresis is performed and rocket-shaped precipitates are formed. The quantitation is based on measuring the height of the precipitate peak.