We describe the changes in extracellular superoxide dismutase (EC-SOD) following cerebral ischemia in mice. Mice were subjected to transient forebrain ischemia and reperfusion. The measurements of EC-SOD using ELISA showed increased brain EC-SOD after 24 h of reperfusion. The immunohistochemical examination showed that EC-SOD immunoreactivity in cortical and striatal capillary wall was conspicuous after 3 h. EC-SOD immunoreactivity was also noted in cortical neurons after 24 h. Northern blot analysis showed an increased EC-SOD mRNA expression in the brain after 24 h. In situ hybridization study demonstrated no mRNA expression of EC-SOD following ischemia and reperfusion in the capillary wall. These findings suggest that serum EC-SOD might accumulate on brain endothelial cells, while cortical neurons produce EC-SOD themselves after cerebral ischemia with reperfusion. © Springer-Verlag 2003.
CITATION STYLE
Fukui, S., Nawashiro, H., Ookawara, T., Suzuki, K., Otani, N., Ooigawa, H., & Shima, K. (2003). Extracellular superoxide dismutase following cerebral ischemia in mice. Acta Neurochirurgica, Supplementum, (86), 83–85. https://doi.org/10.1007/978-3-7091-0651-8_19
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