Spectroscopic Estimation of Cefepime by using Batch, Cloud Point extraction and Flow Injection Analysis methods

Abstract

New simple sensitive spectrophotometric methods are developed for determination of cefepime (CFM) in pure and Pharmaceutical Formulations. The first method conversion primary amine to azo-dye by react cefepime with sodium nitrite and hydrochloric acid followed by coupling with 2,6-dimethylphenol in alkaline medium to obtain a stable reddish-orange colored dye at λ max 515nm. The concentration ranges 2-50 μg / mL, Beer's law is obeyed, correlation coefficient was 0.9996, molar absorptivity was 0.826×104L.mol-1.cm-1 and the detection limit was 0.192 μg/mL. Cloud point extraction (CPE) second method to estimation a trace amount in aqueous solution product from diazotization and measuring with a UV-visible spectrophotometer at λ max 515 nm. The concentration range obeyed the Beer's law was 0.25-10 μg / mL, correlation coefficient was 0.9997, molar absorptivity was 1.169×105L.mol-1.cm-1, detection limit was 0.027 μg/mL, Pre-concentration factor was 25 and Distribution coefficient(D) was 332.35. Last methods flow injection analysis it's simple for estimation the cefepime .The concentration ranges 1-150 μg / mL, Beer's law is obeyed, correlation coefficient was 0.9997, molar absorptivity was 0.341×104L.mol-1.cm-1 and the detection limit was 0.464μg/mL. The proposed methods were successfully, applied to the determination cefepime in pharmaceutical formulation.