Effect of piperine on pharmacokinetics of rifampicin and isoniazid: Development and validation of high performance liquid chromatography method

Abstract

In the present investigation a method for simultaneous determination of rifampicin, isoniazid and piperine by liquid chromatography has been developed and optimized in terms of specificity, accuracy, precision, sensitivity and stability. The purpose of this investigation concerns the effect of piperine on the pharmacokinetic of rifampicin and isoniazid in rat model. Suspension of rifampicin, isoniazid and piperine were administered orally in Swiss albino rat and the drug concentration in plasma was estimated by high performance liquid chromatography. The chromatic separation of rifampicin, isoniazid and piperine were achieved by carrying out on a Zorbax Eclipse XDP C18 column (150 mm × 4.6 mm × 5 μm) using potassium dihydrogen phosphate (pH 4.5) and acetonitrile as the mobile phase (30:70) at a flow rate of 0.8 mL/min with detection at 270 nm. After oral administration the observed pharmacokinetic parameter of rifampicin along with piperine indicates significant enhancement in Cmax and area under the curve (AUC), while in isoniazid with piperine shows reduced Cmax and enhanced-AUC. The observed pharmacokinetics data after oral administration of rifampicin along with piperine indicate significant enhancement in Cmax and AUC and thus bioavailability. The bioavailability of isoniazid was reduced when it was co-administered with piperine, it could be due to delay in gastric emptying time.