The measurement and manipulation of cytosolic free Ca2+ of neutrophils is crucial for investigating the mechanisms within living neutrophils which generate Ca2+ signals and the cellular responses triggered by them. Optical methods for this are the most applicable for neutrophils and are discussed here, especially the use of fluorescent indicators of Ca2+ and photoactivation of reagents involved in Ca2+ signaling. Both of these synthetic agents can be loaded into neutrophils as lipid-soluble esters or can be microinjected into the cell. In this chapter, we will outline some of the techniques that have been used to monitor, visualize, and manipulate Ca2+ in neutrophils. © 2014 Springer Science+Business Media, LLC.
CITATION STYLE
Hallett, M. B., Al-Jumaa, M., & Dewitt, S. (2014). Optical methods for the measurement and manipulation of cytosolic calcium signals in neutrophils. Methods in Molecular Biology, 1124, 107–120. https://doi.org/10.1007/978-1-62703-845-4_8
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