Fed-batch culture of E. coli B was carried out to obtain high concentration of biomass. After requirement of oxygen was met by sparging the pure oxygen, physiological constraints were delineated. High partial pressure of CO2 caused the decrease of the maximum specific growth rate, whereas fermentative byproducts caused the decrease of biomass yield as well as the maximum specific growth rate. © 1987 Kluwer Academic Publishers.
CITATION STYLE
Pan, J. G., Rhee, J. S., & Lebeault, J. M. (1987). Physiological constraints in increasing biomass concentration of Escherichiacoli B in fed-batch culture. Biotechnology Letters, 9(2), 89–94. https://doi.org/10.1007/BF01032744
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