Production of recombinant proteins in bacterial hosts often produces insoluble intracellular particles called inclusion bodies. Recovery of active protein from inclusion bodies generally requires their solubilization in chemical denaturants followed by a refolding strategy. The solubilization is carried out with shaking/stirring and takes several hours.
CITATION STYLE
Datta, I., Gautam, S., & Gupta, M. N. (2013). Microwave assisted solubilization of inclusion bodies. Sustainable Chemical Processes, 1(1). https://doi.org/10.1186/2043-7129-1-2
Mendeley helps you to discover research relevant for your work.