Chromatin immunoprecipitation assays.

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Abstract

Chromatin immunoprecipitation (ChIP) is a powerful tool to study protein-DNA interaction and is widely used in many fields to study proteins associated with chromatin, such as histone and its isoforms and transcription factors, across a defined DNA domain. Here, we show the step-by-step methods currently used in our lab to immunoprecipitate the formaldehyde crosslinked chromatin and further analyze the immuprecipitated DNA by semiquantitative PCR.

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Yan, Y., Chen, H., & Costa, M. (2004). Chromatin immunoprecipitation assays. Methods in Molecular Biology (Clifton, N.J.), 287, 9–19. https://doi.org/10.1385/1-59259-828-5:009

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