Metabolic Flux Analysis in Streptomyces Coelicolor: Effect of nitrogen source

Abstract

Metabolic flux analysis was applied to Streptomyces coelicolor growth under nitrogen limitation with nitrate or ammonia as the nitrogen source. In all cases, maximum specific growth rates corresponding to the same specific glucose uptake rates were calculated in addition to the fluxes of some 200 metabolic reactions. Use of nitrate resulted in lower specific growth rates compared to ammonia. Excretion of some organic metabolites was observed in both limitation cases.