Liquid Chromatographic Determination of Vanillin and Related Aromatic Compounds

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Abstract

This paper describes a reversed-phase liquid chromatographic method for the determination of vanillin, associated natural aromatic compounds and/or synthetic precursors, ethyl vanillin, and coumarin, a commonly encountered adulterant in nonbeverage and beverage alcohol products using a ternary gradient mobile phase. The compounds were separated on a Nova-Pak C18 column by using water, methanol, and tetrahydrofuran as the mobile phase. Measurements were made by using a photodiode array detector at 275 nm. The choice of the mobile phase and the column provides baseline resolution of vanillin and the associated aromatic compounds commonly found in vanilla-flavoring material. Because this method provides low-level detection/quantitation, it is suitable for the characterization of vanilla flavoring materials that are currently added to vanilla flavored beverage alcohol products.

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Jagerdeo, E., Passetti, E., & Dugar, S. M. (2000). Liquid Chromatographic Determination of Vanillin and Related Aromatic Compounds. Journal of AOAC International, 83(1), 237–240. https://doi.org/10.1093/jaoac/83.1.237

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