Teaching old indicators even more tricks: Binding affinity measurements with the guest-displacement assay (GDA)

Abstract

A simple change has important consequences: The guest-displacement assay (GDA) is introduced which allows for binding affinity determinations of supramolecular complexes with spectroscopically silent hosts and guests. GDA is complementary to indicator-displacement assay for affinity measurements with soluble components, but is superior for insoluble or for weakly binding guests.