In vitro methods to study RNA interference during an adenovirus infection.

Abstract

RNA interference (RNAi) has attracted a lot of interest during recent years as a method to knock-down gene expression and as a possible antiviral system. Here we present a collection of in vitro methods to study RNAi and the effect of an adenovirus infection on RNAi. We describe methods to measure the two key enzymatic complexes involved in RNAi: Dicer and RISC.