Molecular signaling through both estrogen and microRNAs are critical for breast cancer development and growth. The activity of estrogen is mediated by transcription factors, the estrogen receptors. Here we describe a method for robust characterization of estrogen-regulated microRNA profiles. The method details how to prepare cells for optimal estrogen response, directions for estrogen treatment, RNA extraction, microRNA large-scale profiling, and subsequent confirmations.
CITATION STYLE
Katchy, A., & Williams, C. (2016). Expression profiles of estrogen-regulated microRNAs in breast cancer cells. In Methods in Molecular Biology (Vol. 1366, pp. 373–393). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3127-9_30
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