Nicotinamide adenine dinucleotide (NAD+) plays a critical role in regulating numerous biological and physiological pathways including metabolism, inflammation, cancer, and aging in mammals. Here we describe a highly quantitative method with reverse-phase high-performance liquid chromatography (HPLC) for the determination of NAD+ levels in cells and tissues. This methodology provides accurate, reliable, and reproducible results of NAD+ measurement, which enables us to analyze various pathophysiological changes in NAD+ levels in vitro and in vivo. © 2013 Springer Science+Business Media, LLC.
CITATION STYLE
Yoshino, J., & Imai, S. I. (2013). Accurate measurement of nicotinamide adenine dinucleotide (NAD+) with high-performance liquid chromatography. Methods in Molecular Biology, 1077, 203–215. https://doi.org/10.1007/978-1-62703-637-5_14
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