Intercellular communication often involves phosphorylation of signal transduction proteins, including mitogen-activated protein kinases (MAPKs). Immunological detection of phosphorylated MAPK can be used to monitor signaling in vivo, identify novel pathway components, and assess ligand activity. In this chapter, I describe a cell co-culture method to assess activity of cell-bound extracellular ligands that result in phosphorylation of the ERK (extracellular signal-regulated kinase) MAPK in Drosophila. This protocol may be adaptable to other pathways and/or model systems.
CITATION STYLE
Steinhauer, J. (2017). Co-culture activation of MAP kinase in Drosophila S2 cells. In Methods in Molecular Biology (Vol. 1487, pp. 235–241). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6424-6_17
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