The gene gun technology can not only be employed for efficient transfer of gene vaccines into upper layers of the skin, but also for application of protein antigens. As a tissue rich in professional antigen presenting cells, the skin represents an attractive target for immunizations. In this chapter we present a method for delivery of the model antigen ovalbumin into the skin of mice termed epidermal antigen incorporation and describe in detail how antigen-specific proliferation in draining lymph nodes can be followed by flow cytometry. © 2013 Springer Science+Business Media, LLC.
CITATION STYLE
Scheiblhofer, S., Ritter, U., Thalhamer, J., & Weiss, R. (2013). Protein antigen delivery by gene gun-mediated Epidermal Antigen Incorporation (EAI). Methods in Molecular Biology, 940, 401–411. https://doi.org/10.1007/978-1-62703-110-3_29
Mendeley helps you to discover research relevant for your work.