ANTI-CANCER POTENCY AND SUSTAINED RELEASE OF PHYTOSOMAL DIALLYL DISULFIDE CONTAINING METHANOLIC ALLIUM SATIVUM EXTRACT AGAINST BREAST CANCER

Abstract

Vesicular drug delivery system like phytosomes is a widely adopted section of pharmaceutical inventions. It improves therapeu tic efficacies of drug by controlled and sustained action. It is also used to improve the therapeutic index, solubility, stability and rapid degradation of drug molecules. Phytosome is made of phytoconstituents of herbal extract that is surrounded and bounded by one or more concentric spheres of lipid layers. The purpose of this study is to synthesize an economical phytosome which will also be an effective alternative to the current medications of cancer. Methanolic extract of Allium sativum containing Diallyl disulphide along with other phenolic compounds were used for the preparation of phytosome as it has the ability to cure and prevent the growth and division of cancer cells. Bioactive compounds were examined by phytochemical analysis. Antioxidant activity of the extract was carried out by DPPH assay that showed that the extract was rich in antioxidants. Presence of Diallyl disulfide having the anti-cancer activity was confirmed by HPLC and GC-MS analysis. The surface morphology and the functional groups of the prepared phytosomal complex were studied by SEM and FTIR analysis respectively. The prepared phytosome showed 100% toxicity against the cancer cell line (MCF 7) at 108.5 μg/ml. Hence, we claim that the Diallyl disulphide containing methanolic Allium sativum encapsulated in phytosome can be an effective alternate for the cancer therapies and this work can also be extrapolated to active targeting of tumour site by attaching the targeting moiety on the surface of the phytosome.