Method development and validation of ondansetron in bulk and pharmaceutical dosage form by stability-indicating RP-HPLC method

Abstract

A precise and feasible high-performance liquid chromatographic (HPLC) method for the analysis of antiemetic drug Ondansetron tablet dosage form has been developed. The analysis was carried out on a zodiac C18 (4.6 x 250mm, 5 mm) column, using a buffer (pH 2.2), acetonitrile (73:27) as the mobile phase using a low pressure gradient mode with flow rate at 1ml/min and analysis was performed at wavelength 246 nm using Photo Diode Array (PDA) detector at ambient temperature. The injection volume was 20μl.The retention time of the drug was 5.2 min. The method produced linear responses in the concentration range of 2.5 to 75μg/ml of Rabeprazole. The LOD and LOQ values for HPLC method were found to be 0.9856 and 2 μg/ml respectively.