Temporal control of the Cre recombinase in transgenic mice by a tetracycline responsive promoter

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Abstract

Gene-targeted mice derived from embryonic stem cells are a useful tool to study gene function during development. However, if the mutation is embryonic lethal and the gene is deleted from the onset of development; later functions in adult animals cannot be studied. Recently, the bacterial Cre-IoxP site-specific recombinat/on system has successfully been used in transgenic animals to produce tissue-specific and temporal deletions. We have evaluated the tetracycline responsive binary system for its ability to transiently express the Cre recombinase in transgenic mice. In this system, a transactivator fusion protein composed of the tetracycline repressor (tetR) and the acidic domain of the herpes simplex viral protein 16 (VP16) can regulate the expression of the Cre gene from a promoter containing tet-operator (tetO) sequences. In the absence of tetracycline, the Cre gene is expressed and will induce site-specific recombination between two IoxP sites. In the presence of tetracycline, the Cre gene will not be expressed and recombination will not occur.

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St-Onge, L., Furth, P. A., & Gruss, P. (1996). Temporal control of the Cre recombinase in transgenic mice by a tetracycline responsive promoter. Nucleic Acids Research, 24(19), 3875–3877. https://doi.org/10.1093/nar/24.19.3875

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