Strategies to maximize recombinant protein expression in Maize Kernels

Abstract

Recombinant DNA technology has been used to add new functionalities to maize seed. Some of these added functionalities, such as the production of pharmaceuticals or the introduction of enzyme pathways to increase nutritional value, rely on the efficient expression of heterologous proteins. Maize is an exemplary system for the production of recombinant proteins, particularly those that are used for human and veterinary health, because it is a safe and abundant crop. This chapter reviews some of the traits that make maize a popular choice for recombinant protein production, assesses the various factors that contribute to the high-level expression of heterologous proteins, and analyzes examples of successful approaches. It also considers strategies to minimize the inadvertent mixing of recombinant plants with food and feed streams, and the unintended escape of genetically engineered germplasm.