This chapter describes the topic of gene therapy based on colloidal drug delivery, as an alternative to the use of viral carriers. Non-viral vectors are promising transfection agents and do not suffer from limitations related to toxicity and immunogenic effects. In particular, lipid-based aggregates are generally considered biocompatible and versatile nanocarriers whose composition can be designed to include a cationic molecule which ensures strong interaction with nucleic acid. Herein the main issues related to complex formation and in vitro administration are illustrated with key examples, such as liposome-DNA plasmid (pDNA) association and micelles-siRNA complexes.
CITATION STYLE
Falsini, S., & Ristori, S. (2016). Lipoplexes from non-viral cationic vectors: DOTAP-DOPE liposomes and gemini micelles. In Methods in Molecular Biology (Vol. 1445, pp. 33–43). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3718-9_3
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