A New Floating Cell Line (OAT) Derived from Human Pulmonary Cancer of Oat Cell Type

Abstract

A cell line was newly established from a lymph node with metastasis from oat cell carcinoma of the lung. The culture medium consisted of RPMI-1640 supplemented with 20% fetal bovine serum. The culture cells grew floating free in the culture fluid and have been serially subcultured for 80 generations, up to date. Doubling time was approximately 24 hours. Multi-lobulated nuclei and well-developed Golgi apparatus were prominent and secretory granules were occasionally observed. No virus particles were found. Repeated examination of immunofluorescence revealed no evidence of immunoglobulin synthesis and EB virus production. Chromosomal analysis of cultured cells of the 8th generation revealed the modal number of 47 chromosomes and the karyotypic anomaly of B5q+, t (A1q+; Cq-) in all of the mitotic cells. These characteristics of this cell line obviously differ from the lymphoblastoid cell lines derived from the human lymphoid tissues, strongly supporting the possibility of its cancer cell origin. This cell line, hence, was designated as OAT line after oat cell carcinoma. © 1971, The Japan Lung Cancer Society. All rights reserved.