Agrobacterium tumefaciens-mediated transformation is an effective method for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an Agrobacterium strain carrying a standard binary vector. In addition to step-by-step laboratory transformation procedures, we include extensive details in growing donor plants and caring for transgenic plants in the greenhouse.
CITATION STYLE
Frame, B., Warnberg, K., Main, M., & Wang, K. (2015). Maize (Zea mays L.). Methods in Molecular Biology, 1223, 101–117. https://doi.org/10.1007/978-1-4939-1695-5_8
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