This method describes the combination of chemical cross-linking and high-resolution mass spectrometry for analyzing conformational changes in target proteins that are induced by drug binding. Our approach is exemplified for detecting conformational changes within the peroxisome proliferator-activated receptor alpha upon binding of low-molecular weight compounds, proving that our strategy provides a basis to efficiently characterize target protein-drug interactions. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Müller, M. Q., & Sinz, A. (2012). Chemical cross-linking and high-resolution mass spectrometry to study protein-drug interactions. Methods in Molecular Biology, 803, 205–218. https://doi.org/10.1007/978-1-61779-364-6_14
Mendeley helps you to discover research relevant for your work.