Polyclonal expansion of human CD4(+)CD25(+) regulatory T cells.

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Abstract

Based on results from experimental animal models, the adoptive transfer of CD4(+)CD25(+)FOXP3(+) regulatory T cells (Treg) is expected to be efficacious in treating autoimmune and inflammatory diseases, as well as in preventing alloresponses after solid organ or stem-cell transplantation. For potential clinical applications, large numbers of Treg cells in maximum purity will be required to avoid the risk of disease exacerbation by contaminating effector T cells. We have recently described methods for the efficient in vitro expansion of human Treg cells and identified CD4(+)CD25(high)CD45RA(+) T cells as the ideal starting population for the generation of homogeneous and stable Treg cell products. Here, we provide detailed instructions for their identification, isolation, expansion, and functional characterization.

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Hoffmann, P., Eder, R., & Edinger, M. (2011). Polyclonal expansion of human CD4(+)CD25(+) regulatory T cells. Methods in Molecular Biology (Clifton, N.J.), 677, 15–30. https://doi.org/10.1007/978-1-60761-869-0_2

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