Agrobacterium tumefaciens-mediated transformation (ATMT) has become an important tool for functional genomics in fungi. ATMT-based approaches such as random insertional mutagenesis and targeted knockout are widely used for gene functional analysis in plant-pathogen interactions. Here, we describe a protocol for the identification of pathogenicity and virulence genes through random insertional mutagenesis using the fungal wilt pathogen Verticillium dahliae as an example for the protocol. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Santhanam, P. (2012). Random insertional mutagenesis in fungal genomes to identify virulence factors. Methods in Molecular Biology, 835, 509–517. https://doi.org/10.1007/978-1-61779-501-5_31
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