S-nitrosylation, which involves the coupling of an NO group to the reactive thiol of Cys residue(s) in a polypeptide, is an important posttranslational modification detected in a variety of proteins. Here, we present the S-nitrosylation of recombinant galectin-2 (Gal-2) using S-nitrosocysteine and the measurement of the molecular ratio of S-nitrosylation of Cys residues in the Gal-2 protein.
CITATION STYLE
Tamura, M., & Arata, Y. (2020). Expression, S-Nitrosylation, and measurement of S-Nitrosylation ratio of recombinant galectin-2. In Methods in Molecular Biology (Vol. 2132, pp. 55–63). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0430-4_6
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