Proline accumulation, protein pattern and photosynthesis in Bacopa monniera regenerants grown under NaCl stress

Abstract

Shoots of Bacopa monniera exhibited 100% regeneration on Murashige and Skoog medium with 2% sucrose, 0.2 mg dm-3 1-naphthaleneacetic acid, 0.5 mg dm-3 6-benzylaminopurine and 50 mg dm-3 glutamine. When the medium was supplied with various concentrations (5 - 15 g dm-3) of sodium chloride, proline content in regenerants was six times higher than in the control. With increasing NaCl concentration photosynthetic rate decreased and fresh mass and root length of regenerants declined. NaCl also induced formation of new proteins.