CRISPR/Cas9-based gene targeting allows deletion of a gene of interest from cultured cell lines. Due to difficulty in transiently transfecting hematopoetic cells with components required for this process, we have adopted a lentiviral system for delivery of the CRISPR/Cas9 components into myeloid cell lines. Here, we detail the process of knocking out genes from pools of cultured myeloid cells using this CRISPR/Cas9 system and describe methods of validating these knockout pools.
CITATION STYLE
Baker, P. J., & Masters, S. L. (2018). Generation of genetic knockouts in myeloid cell lines using a lentiviral CRISPR/Cas9 system. In Methods in Molecular Biology (Vol. 1714, pp. 41–55). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7519-8_3
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