Although recent molecular biology advances provide a very effective way in determining localized gene expression, the visualization of the expression by in situ hybridization of whole-mount embryos or paraffin-embedded tissue sections continues to be an excellent method to determine overall gene expression. In this chapter, I review protocols designed to determine mRNA expression by in situ hybridization techniques, in particular, focusing on the developing stratified epidermis.
CITATION STYLE
Morasso, M. I. (2010). Detection of gene expression in embryonic tissues and stratified epidermis by in situ hybridization. Methods in Molecular Biology (Clifton, N.J.), 585, 253–260. https://doi.org/10.1007/978-1-60761-380-0_17
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