Protocols for Establishing Fungi-Protocorm Culture

Abstract

Establishing fungi-protocorm culture requires the isolation, identification, and in vitro germination test of symbiotic fungi. In situ seed baiting is a useful technique to attract and isolate seed germination-promoting fungi. In this chapter, a protocol for establishing protocorm-fungi culture using a seed baiting technique is detailed. This technique enables the identification of potential mycorrhizal fungi specifically at the time of seed germination and during protocorm development.