The mammalian hair follicle epithelial component contains various lineages of keratinocytes as well as their progenitor/stem cells. To characterize the subpopulations contained within this component and assess their functional capacity, the development of a feasible method to isolate them is greatly needed. In this chapter we describe a standard method by which a small subset of human follicular keratinocytes can be isolated for subsequent analysis. Detailed methods for enzymatic digestion of fresh human scalp, flow cytometry, cultivation of sorted cells and a colony forming assay are described. © 2013 Springer Science+Business Media, LLC.
CITATION STYLE
Inoue, K., & Yoshimura, K. (2013). Isolation and characterization of human hair follicle epithelial cells. Methods in Molecular Biology, 946, 411–421. https://doi.org/10.1007/978-1-62703-128-8_26
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