Development of a Rapid Method for Determination of Main Higher Alcohols in Fermented Alcoholic Beverages Based on Dispersive Liquid-Liquid Microextraction and Gas Chromatography-Mass Spectrometry

Abstract

Higher alcohols are important flavor compounds in alcoholic beverages. A new method was developed to determinate the main higher alcohols based on dispersive liquid-liquid microextraction (DLLME) coupled with GC/MS. Huangjiu was selected as an example to develop the method. The optimal DLLME parameters were acquired: the sample was diluted one time with water, no pH adjustment was required, 600 μL dichloromethane as extraction solvent, 1.5 mL acetonitrile as dispersant, 3.5 mL of sample volume, 1 min of extraction time and no salt addition. The performance of the method was satisfactory. The results obtained from DLLME were consistent with those from head space solid phase microextration (HS-SPME) and static headspace (SHS). In addition, DLLME is simpler and more time-saving compared with HS-SPME and SHS. These results indicated that the present method is satisfactory and suitable for determination of higher alcohols in Huangjiu, especially for routine inspection and batch analysis.