Effects of Pressure and Magnetic Field on Glutathione Production by Saccharomyces cerevisiae

Abstract

Saccharomyces cerevisiae (S. cerevisiae) has been studied as a model microorganism to understand the mechanism of glutathione (GSH) production. Furthermore, magnetic field (MF) application is a novel approach to stimulate microorganism metabolism to increase growth and produce more biomolecules. This study aimed to evaluate whether GSH production by S. cerevisiae ATCC 7754 would be affected by different pressure levels and MF application in an airlift bioreactor. Influence of pressure (0.0 - 0.15 MPa), medium recycle velocity (3.0 and 15.0 cm s-1) and MF application (0.0 and 3.0 mT) was evaluated. Biomass and GSH concentrations at high pressure and medium recycle velocity were higher than the ones in the control, whereas MF decreased biomass and GSH concentrations. The best results of biomass concentration and GSH production were 17.79 g L-1 and 162.92 mg L-1, respectively. at 0.05 MPa, 15.0 cm s-1 and without any MF application. Results of the pressured airlift bioreactor showed that this kind of reactor may be used for producing GSH and for reducing energy costs.